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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized <t>Pam</t> <t>3</t> <t>CSK</t> <t>4</t> at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.
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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized <t>Pam</t> <t>3</t> <t>CSK</t> <t>4</t> at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.
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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized <t>Pam</t> <t>3</t> <t>CSK</t> <t>4</t> at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.
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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized <t>Pam</t> <t>3</t> <t>CSK</t> <t>4</t> at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.
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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized <t>Pam</t> <t>3</t> <t>CSK</t> <t>4</t> at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.
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pam 2 csk 4  (InvivoGen)
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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized <t>Pam</t> <t>3</t> <t>CSK</t> <t>4</t> at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.
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Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized Pam 3 CSK 4 at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.

Journal: bioRxiv

Article Title: Preclinical antiviral study of a liver-targeted TLR1/2 agonist in an immune-competent mouse model of HBV infection

doi: 10.64898/2026.05.27.728102

Figure Lengend Snippet: Six-weeks-old C57BL/6 male mice were mock-transduced or transduced with 10 11 ⍰vge of AAV-HBV 1.3 mer genotype D/serotype ayw for 31 days to allow establishment of chronic infection. At day-35, treatment cycles were started, after mice were evenly grouped according to viremia and HBsAg antigenemia. Mice (n⍰= ⍰7/group) were injected, at indicated days with vehicle (PBS), free/non vectorized Pam 3 CSK 4 at 100 μg/injection, NP-Pam 3 CSK 4 at 5 μg/shot, or NP-Pam 3 CSK 4 at 20 μg/shot. 3TC was administrated continously at approximately 100 mg/kg/day (in drinking water) between days-35 and 64 and days-79 and 90. Blood/serum was collected at indicated times (blood drop symbol). Mice were euthanized at 90 days post-transduction. The different readouts and used techniques for exploitation of blood and liver samples are indicated.

Article Snippet: Either Pam 3 CSK 4 (InvivoGen, USA) or the near-infrared fluorescent probe DiR XenoLight Dye (λ excitation = 750 nm, λ emission = 770 nm) (Perkin Elmer, USA) was incorporated during the nanoprecipitation.

Techniques: Transduction, Infection, Injection